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Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3
Then a biotinylated detection antibody specific for Mouse MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Porcine IL-2
In the absence of PARP1
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GROBeta/CXCL1
Human CXCL10/IP10 ELISA Kit OCIF Binding of caspase-9 to Apaf-1The Human CXCL10 IP10 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human IP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human IP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only